Nanoscale monitoring of mitochondria and lysosome interactions for drug screening and discovery

Nanoscale monitoring of mitochondria and lysosome interactions for drug screening and

discovery

Qixin Chen;Kai Zhang;Zijian Guo;Jiajie Diao;Xintian Shao;Zhiqi Tian;Yang Chen;Payel Mondal;Fei Liu;Fengshan Wang;Peixue Ling;Weijiang He

【期刊名称】《纳米研究（英文版）》 【年(卷),期】2019(012)005 【摘要】Technology

advances

in

genomics,proteomics,and

metabolomics largely expanded the pool of potential therapeutic targets.Compared with the in vitro setting,cell-based screening assays have been playing a key role in the processes of drug discovery and development.Besides the commonly used strategies based on colorimetric and cell viability,we reason that methods that capture the dynamic cellular events will facilitate optimal hit identification with high sensitivity and specificity.Herein,we propose a live-cell screening strategy using structured illumination microscopy (SIM) combined with an automated cell colocalization analysis software,CellprofilerTM,to screen and discover drugs for mitochondria and lysosomes interaction at a nanoscale resolution in living cells.This strategy quantitatively benchmarks

the

mitochondria-lysosome

interactions

such

as

mitochondria and lysosomes contact (MLC) and mitophagy.The automatic quantitative analysis also resolves fine changes of the