Molecular Cloning and Expression Analysis of Cryptochrome Gene Ps CRY2in Tree Peony

Molecular Cloning and Expression Analysis of Cryptochrome Gene Ps CRY2in Tree Peony

REN Xiuxia;WANG Shunli;XUE Jingqi;ZHU Fuyong;LIU Chuanjiao;ZHANG Xiuxin;

【期刊名称】《园艺学报：英文版》 【年(卷),期】2016(000)006

【摘要】Cryptochromes are blue/ultraviolet-A(UV-A) light receptors involved in regulating various aspects of plant growth and development.Investigations of the structure and functions of cryptochromes in plants have largely focused on herbaceous plants. However, few data on the function of CRY2 are available in woody plants. In this study, a cryptochrome 2(CRY2) gene was isolated from Paeonia suffruticosa by Reverse Transcription Polymerase Chain Reaction(RT-PCR). Sequence alignment and motif analysis showed that the deduced amino acids contained a PHR domain near the amino terminus and a CCT domain near the carboxy terminus. Ps CRY2 showed high identity with At CRY2 of Arabidopsis.Phylogenetic analysis indicated that it was closely related to Citrus sinensis. Gene expression analysis revealed that the highest expression levels of Ps CRY2 occurred in the bud and seed embryo of P. suffruticosa, followed by the roots, stems, and leaves. Ps CRY2 was upregulated during the entire process of bud differentiation, whereas this was downregulated during the early stage of bud development and upregulated in the middle and late