Propofol enhances the field excitatory postsynaptic potentials in CA1 hippocampal slices o

Propofol enhances the field excitatory postsynaptic potentials in CA1 hippocampal slices of young and

aged mice

Yin Yiqing;William J Middleton;Carlos M.Florez;Peter L.Carlen;Hossam EI-Beheiry

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2014(127)001

【摘要】Background Increasing age was shown to decrease the requirements for propfol.However,the mechanisms of ageing-induced potentiation of anesthetic actions have not been clearly explored.The aim of this study is to compare the effects of propofol on the field excitatory postsynaptic potentials (fEPSPs) in hippocampal slices of young and aging mice.Methods Brain slices were prepared from C57BL6 male young (2 months) and aging (＞12 months) mice.The dendritic field excitatory postsynaptic potential was recorded from the CA1 stratum radiatum using patch clamp electrophysiological methods.A bipolar concentric stimulating electrode was placed along the Schaffer collateral for othodromic stimulation.The effects of clinically-relevant concentrations of propofol were studied in the young and ageing mouse

tissues.Results

Propofol

application

increased

the

orthodromically evoked fEPSP produced in slices taken from young and older animals.A striking feature in the I/O relationship was the decreased enhancement of the fEPSPs by propofol in slices from older