ZMW型环氧乙烷灭菌箱对异体骨材料灭菌效果的初步观
察
孙建华;史晨辉;董卫;黄新玲;王永明;董金波;刘维刚
【期刊名称】《中国组织工程研究》 【年(卷),期】2002(006)002
【摘要】@@ Background: The foreign bone material of the same species was widely applied, while for the multiple manipulating processes, it got lots of chances to be infected, especially for the bone manufacturing, the pollution odds increased greatly, so the sterilizing process was most important. Objective: To observe the sterilizing effects of ZMW anprolene case on foreign bone materials to find a safe and economic disinfecting way. Unit:First attached hospital of the Shihezi medical university. Subject:The manipulating method of the foreign bone material of the same species: In clean environment., bone was got in 2 hours after death of the donor, soft tissue was removed and it was made to foreign bone material of 0.5 cm× 0.5 cm× 2 cm, it was washed with water and marinated till the water got clear, then washed with distilled water repeatedly, lipid was removed with 1:1 chloroform and methanol for 4 hours at room temperature, calcium was removed with 0.6 mol/L HCL for 4 hours at 4 ℃ , it was washed with distilled water till the pH was 6.5. The bone material after treating were divided into two groups, one was dried in common air dried to make wet one plantation
ZMW型环氧乙烷灭菌箱对异体骨材料灭菌效果的初步观察
