HPLC同时测定风寒感冒颗粒中葛根素和升麻素苷的含量
汤云;岳宁;刘宏明
【期刊名称】《食品与药品》 【年(卷),期】2017(019)004
【摘要】Objective To establish an HPLC method for simultaneous determination of puerarin and prim-O-glucosylcimifugin in Fenghan Ganmao Granules. Methods HPLC with gradient elution was performed on an Agilent ZORBAX SB-C18 column (250 mm×4.6 mm, 5 μm), using acetonitrile-0.1 ％ phosphoric acid as mobile phase. The column temperature was 40 ℃. Results The good linearity was achieved in the range of 0.04070-2.0350 μg for puerarin and in the range of 0.0008176-0.04088 μg for prim-O-glucosylcimifugin. The average recoveries were 100.4 ％ and 101.1 ％, with RSD of 1.3 ％ and 1.2 ％, respectively. Conclusion This method is accurate, sensitive and reproducible, and can be used for the overall quality control for Fenghan Ganmao Granules.%目的 建立高效液相色谱法(HPLC)同时测定风寒感冒颗粒中葛根素和升麻素苷含量的方法.方法 采用Agilent ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5μm),乙腈-0.1％磷酸梯度洗脱,柱温40℃.结果 在0.04070～2.0340μg范围内,葛根素进样量与峰面积呈良好线性关系,Y=5270.2X-10.331(r=1.000);在0.0008176～0.04088μg范围内,升麻素苷进样量与峰面积呈良好线性关系,Y=16003.6X-6.765(r=0.9999);平均回收率分别为100.5％和101.1％,RSD分别为1.3％和1.2％.结论 此法准确,灵敏度高,重复性好,为风寒感冒颗粒全面的
HPLC同时测定风寒感冒颗粒中葛根素和升麻素苷的含量
