激光共聚焦显微镜操作手册最终版 - 图文

Nikon A1激光共聚焦显微镜 软件操作指南

徐珏 2011.12.3 17:59 于苏州宝石御景园

目录

标准探测模式（DU4）图像拍摄流程 ........................................................................................... 1 一、软件开启 ................................................................................................................................... 1 二、基本设置 ................................................................................................................................... 2

[A1 Setting]界面主要功能区域简介 ...................................................................................... 2 [Setting]界面功能简介 ............................................................................................................ 4 [Filter and Dye]功能区简介 .................................................................................................... 6 串色现象解释及Line模式图解 ............................................................................................ 6 Line模式图解 ......................................................................................................................... 7 [scan setting]功能区简介 ........................................................................................................ 7 三、获得图像 ................................................................................................................................... 8

[Acquisition]功能区简介 ...................................................................................................... 10 放大方式选择按钮简介 ........................................................................................................ 13 图片扫描参数调用简介 ........................................................................................................ 17 JP2格式图片转化为JPG/TIFF格式图片的操作 ............................................................... 18 各通道荧光图像自由叠加的介绍 ........................................................................................ 18 数据分析......................................................................................................................................... 21 一、ROI区域分析 ......................................................................................................................... 21 二、LUTs调节图像荧光亮度 ...................................................................................................... 23 三、去背景..................................................................................................................................... 25 四、标尺添加 ................................................................................................................................. 26

五、一维荧光亮度分布 ................................................................................................................. 27 六、长度面积等常规测量 ............................................................................................................. 28 七、细胞计数 ................................................................................................................................. 28

[Binary Toolbar]界面简介 .................................................................................................... 32 光谱扫描模式（SD模式） ........................................................................................................... 34 一、SD模式基本设置................................................................................................................... 34

[Detector]标签页功能简介 ................................................................................................... 35 [Binng/Skip]标签页简介 ....................................................................................................... 36 二、图像获得 ................................................................................................................................. 37 三、数据分析 ................................................................................................................................. 39

[Spectrum Profile]视窗简介 .................................................................................................. 40 四、光谱拆分 ................................................................................................................................. 40 虚拟滤光扫描模式（VF模式） ................................................................................................... 47 一、VF模式基本设置................................................................................................................... 47

[Detector]设置标签页简介 ................................................................................................... 48 [Gating Setting]标签页简介 .................................................................................................. 48 二、获得图像 ................................................................................................................................. 48 时间序列拍摄 ................................................................................................................................. 49

[Capture Timelapse]视窗简介 ............................................................................................... 49 [ND]界面简介 ....................................................................................................................... 51 [Time Measurement]界面简介 .............................................................................................. 53

Z轴序列拍摄 ................................................................................................................................. 54

[Capture Z-series]视窗简介 .................................................................................................. 55 拼大图拍摄..................................................................................................................................... 60 光活化序列拍摄 ............................................................................................................................. 61

[Photo Acquition]功能区简介 ............................................................................................... 62 [Sequential Stimulation]界面简介 ........................................................................................ 63 常见细胞类型和细胞器形状 ......................................................................................................... 65 常用荧光染料及应用领域 ............................................................................................................. 68