TANDEM REPEATING OF THE EXPRESSION CARTRIDGE—— A NOVEL STRATEGY TO ENHANCE THE EXPRES

TANDEM REPEATING OF THE EXPRESSION CARTRIDGE—— A NOVEL STRATEGY TO ENHANCE THE EXPRESSION EFFICIENCY OF CLONED GENE IN

ESCHERICHIA COLI

林缨; 卢圣栋

【期刊名称】《《中国医学科学杂志：英文版》》 【年(卷),期】1996(011)004

【摘要】A novel strategy to enhance the expression eHiciency of cloned target gene in Escher&hia colt was developed. The whole expression cartridge, consisting of promoter, SD sequence, target gene and transcription terminator, was tandem repeatedly engineered into a expression plasmld. Consequently, the copynumber of specific gene was increased substantially, leading to the improvemem of expression efficiency.Using this approach, a recombinant plasmid, designed as pLYD, was constructed and transformated into the Escherichia coti strain DHSa. Upon induction, the desired protein was synthesized in a considerable level and accumulated up to 63% of the total cell proteins, The pretent study reveated that taildem repeating of expression cartridge provided a convenient means to improve expression level efficiently.

【总页数】5页(204-208)

【关键词】大肠埃希氏菌; 克隆; 基因表达效率; 插入式表达盒