HPLC测定吡嗪酰胺片的含量
毕言锋;仝桂平
【期刊名称】《食品与药品》 【年(卷),期】2013(015)003
【摘要】目的 为吡嗪酰胺片含量测定建立新的质量控制方法.方法 采用高效液相色谱法(HPLC)测定吡嗪酰胺片中吡嗪酰胺的含量.色谱柱:Agilent C18(250 mm×4.6 mm,5μm),流动相:甲醇-水(10∶90),检测波长269 nm,流速1.0mL/min,柱温30℃,理论板数按吡嗪酰胺峰计算应不低于3000.结果 在2.024～20.24 μg/mL浓度范围内,吡嗪酰胺进样量与峰面积呈良好的线性关系,r=0.9999,平均回收率为99.8％(n=6),RSD=0.8％.结论 本法简便、准确,重现性及灵敏度高,可用于吡嗪酰胺片中吡嗪酰胺的含量测定.%Objective To build a new quality control method for pyrazinamide tablets.Methods HPLC was adopted to determine pyrazinamide content in pyrazinamide tablets.Agilent C18 column (250 mm×4.6 mm,5 μm) was used,with methanol∶water (10∶90) as mobile phase.The flow rate was 1.0 mL/min.The detecting wavelength was 269 nm and the column temperature was 30 ℃.The column efficiency determined from the pyrazinamide' s peak was not less than 3000 theoretical plates.Results The linear concentration range for pyrazinamide analysis was 2.024～20.24μg/mL,r=0.9999.The average recovery was 99.8 ％ (n=6),with RSD of 0.78 ％.Conclusion This method is simple and precise,with high repeatability and sensitivity,so it can be used in the content
HPLC测定吡嗪酰胺片的含量
