HIV感染者抗病毒治疗后HIV-1辅助受体的利用情况
屈水令;袁霖;黄洋;徐维四;余小玲;刘玉磊;邢辉;邵一鸣;马丽英
【期刊名称】《中华预防医学杂志》 【年(卷),期】2010(044)011
【摘要】Objective To investigate HIV-1 co-receptor usage in patients experienced anti-retroviral therapy (ART) in Anhui and Henan province of China. Methods A total of 45 HIV-1 infected individuals who have experienced ART and 109 un-experienced ART patients from Anhui and Henan province, which were called as treatment group and treatment-negative group, were selected as study subjects. HIV-1 strains were isolated from peripheral blood mononuclear cells of whole blood from patients. HIV-1 p24 in the culture supernatant was measured using a commercial enzyme-linked immunosorbent assay (ELISA) kit. HIV-1 coreceptor usage was identified using Ghost cell lines expressing CD4 and the chemokine receptor CCR5 or CXCR4. Results Among 45 HIV strains from the treatment group,22 (48.9% ) strains used CCR5 as a coreceptor ( R5 tropic strain) ,21 (46.7% ) strains used CXCR4/CCR5 as a co-receptor ( X4/R5 duel tropic strain) ,and 2(4.4%) used only CXCR4 as a co-receptor( X4 tropic strain). In 109 strains from treatmentnegative group,96 (88.1% ) strains used CCR5 as a co-receptor( R5 tropic strain), 13 ( 11.9% ) strains used CCR5/CXCR4 as a co-receptor use( X4/R5 strain). A significant difference was found between two groups in X4 co-
HIV感染者抗病毒治疗后HIV-1辅助受体的利用情况
