Repression of interferon-γexpression in T cells by prosperorelated Homeobox protein

Repression of interferon-γexpression in T cells by

prosperorelated Homeobox protein

Linfang

Wang;Jianmei

Zhu;Shifang

Shan;Yi

Qin;Yuying

Liu;Yuan

Kong;Jing

Wang;Youhua Xie

【期刊名称】《细胞研究（英文版）》 【年(卷),期】2008(018)009

【摘要】Interferon-gamma (IFN-γ) is a major proinflammatory effector and regulatory cytokine produced by activated T cells and NK cells. IFN-γ has been shown to play pivotal roles in fundamental immunological processes such as inflammatory reactions,cell-mediated immunity and autoimmunity. A variety of human disorders have now been linked to irregular IFN-γ expression. In order to achieve proper IFN-γ-mediated immunological effects,IFN-γ expression in T cells is subject to both positive and negative regulation. In this study,we report for the first time the negative regulation of IFN-γ expression by Prospero-related Homeobox (Prox1). In Jurkat T cells and primary human CD4+ T cells,Proxl

expression

decreases

a

quickly

upon

T in

cell IFN-γ

activation,concurrent with dramatic increase

expression.Reporter analysis and chromatin immunoprecipitation (ChIP) revealed that Proxl associates with and inhibits the transcription activity of IFN-γ promoter in activated Jurkat T cells. Co-immunoprecipitation and GST pull-down assay demonstrated a direct binding between Proxl