肝纤维化早期定量诊断方法研究

肝纤维化早期定量诊断方法研究

姚敏捷;李艳;罗卫国;王瑜;潘自铁

【期刊名称】《吉林医学》 【年(卷),期】2001(022)002 【摘要】Objective

To establish a sensitive,specific and simple

measurement for serum collagen Ⅳ which can be used with HBsAg,HBsAb.HBeAb HBcAg and HbcAb.Method By preparing anti-human type Ⅳ collagen IgG with rabbits and utilizing sodium periodate horse-radish peroxidase-labelling anti-human type Ⅳ collagen monoclonal

antibody

to

change

dilution

component

and

concentration,we established sandwich ELISA.Results ELISA had a lowest testing limit of 30μg/L,the average recovery was 102.75%,CVs of intra-assay were respectively 6.0 and 11.8% and the reference value by testing 50 healthy people was 97.9μg/L±36.3μg/L，the average value of liver cirrhosis type Ⅳ collagen was 254.82μg/L±69.9μ/g.Conclusion The improved method is sensitive,exact,stable and specific with small amount of sample and short testing time.It is easy to spread in clinical practice.%目的：建立一种敏感、特异、操作简单的血清Ⅳ型胶原蛋白(COLⅣ)测定方法，并可与肝炎标记物同步进行测定。方法：以免疫家兔制备抗人COLⅣ多抗包被微孔板，采用改良过碘酸钠法辣根过氧化物酶标记抗人COLⅣ单抗，改变各种稀释液成分及浓度，建立双抗夹心酶免疫测定法(ELISA)。结果：最低检测极限为30μg/L，平均回收率为102.75%，批内和批间变异系数分别