Cardiomyocyte-like differentiation of human bone marrow mesenchymal stem cells after expos

Cardiomyocyte-like differentiation of human bone marrow mesenchymal stem cells after exposure to

5-azacytidine in vitro

Feng CAO;Lili NIU;Ling MENG;Lianxu ZHAO;Dongmei Wang;Ming ZHENG;Cixian BAI;Guoliang JIA;Xuetao PEI

【期刊名称】《老年心脏病学杂志（英文版）》 【年(卷),期】2004(001)002

【摘要】Objective To investigate the potential of adult mesenchymal stem cells (MSCs) derived from human bone marrow to undergo cardiomyogenic differentiation after exposure to 5-azacytidine (5-aza) in vitro. Methods A small bone marrow aspirate was taken from the iliac crest of human volunteers, and hMSCs were isolated by 1.073g/mL Percoll and propagated in the right cell culturing medium as previously described. The phenotypes of hMSCs were characterized with the use of flow cytometry. The hMSCs were cultured in cell culture medium (as control) and medium mixed with 5-aza for cellular differentiation. We examined by immunohistochemistry at 21 days the inducement of desmin, cardiac-specific cardiac troponin I (cTnI), GATA 4 and connexin-43 respectively. Results The hMSCs are fibroblast-like morphology and express CD44+ CD29+ CD90+ / CD34- CD45- CD31- CD11a. After 5-aza treatment, 20-30% hMSCs connected with adjoining cells and coalesced into myotube structures after 14days. Twenty-one days after 5-aza