Preparation of arsenic trioxide-loaded albuminutes immuno-nanospheres and its specific kil

Preparation of arsenic trioxide-loaded albuminutes immuno-nanospheres and its specific killing effect

on bladder cancer cell in vitro

ZHOU Jie;ZENG Fu-qing;LI Chong;TONG Qiang-song;GAO Xiang;XIE Shu-sheng;YU Li-zhang

【期刊名称】《中华医学杂志（英文版）》 【年(卷),期】2005(118)001

【摘要】Background Recently, arsenic trioxide (As2O3) was considered as a novel anti-tumor agent. However, it showed severe toxicity effect on normal tissue at the same time. To improve its therapeutic efficacy and decrease its toxicity,we prepared arsenic trioxide-loaded albuminutes immuno-nanospheres [As2O3-(HAS-NS)-BDI-1] targeted with nonoclonal antibody (McAb) BDI-1 and tested its specific killing effect against bladder cancer cell. Methods As2O3-HAS-NS was prepared by chemical cross-linking method. Monoclonal antibody BDI-1 was purified with ammonium sulphate saltingout and chromatography. Albuminutes microspheres were conjugated with McAb by SPDP cross-linking method.Concentration of As in As2O3- (HAS-NS)-BDI-1 and As2O3-HAS-NS was measured by atomic fluometry method. As2O3- (HAS-NS)-BDI-1 and its activity were detected by SDS-PAGE reduction electrophoresis, indirect immunofluorescence test, light microscope and scanning electron microscope observation. Acridine orange staining and