752紫外可见分光光度计
使用说明书
本说明书详细阐述了仪器的使用方法、故障排除及维护与保养等内容。请在使用前仔细阅读本说明，并请妥善保管以备日后查阅。
制造计量器具许可证编号: 沪制01120041号
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目录
章 节 1 1.1 1.2 1.3 1.4 1.4.1 1.4.2 1.4.3 1.5 1.5.1 1.5.2 22.1 2.2 2.3 2.4 3 3.1 3.2 3.2.1 3.2.1.1 3.2.1.2 3.2.1.3 3.2.2 3.2.2.1 3.2.2.1.1 3.2.2.1.23.2.2.2 3.2.2.3 3.2.3 3.2.3.1 3.2.3.2 3.2.3.2.1 3.2.3.2.2 3.2.3.3 3.2.3.4 3.2.3.5 3.2.3.6 3.2.3.7 3.2.3.8 3.2.3.9 3.2.3.10 3.2.3.11
标题
概况··························································································································原理··························································································································应用··························································································································特点··························································································································主要技术参数和规格································································································仪器技术指标············································································································仪器使用条件············································································································仪器规格···················································································································仪器结构简介···········································································································主机左前视图············································································································主机后视图···············································································································
仪器安装···················································································································标准配置···················································································································安装环境···················································································································供电要求···················································································································安装··························································································································
使用及操作方法········································································································显示与键盘···············································································································仪器操作···················································································································仪器启动和系统自检·································································································仪器启动···················································································································系统自检···················································································································进入光度测量界面····································································································光度测量···················································································································设定工作波长············································································································利用预设的工作波长移动至需要的工作波长·····························································利用< GOTOλ >键移动至需要的工作波长·····························································测量结果显示界面····································································································数据打印···················································································································系统设定···················································································································显示模式···················································································································浓度因子···················································································································输入浓度因子············································································································测定浓度因子············································································································删除数据···················································································································预设工作波长············································································································氘灯··························································································································钨灯··························································································································暗电流校正···············································································································波长校正···················································································································输入换灯波长············································································································恢复出厂设置············································································································版本信息···················································································································
i 页码 11111122223
444456667777888889910111111121212131313141414
目录
（续）
4 应用操作···················································································································4.1 测量样品的透射比或吸光度······················································································4.2 利用浓度因子测量样品的浓度··················································································4.2.1 已知浓度因子测量样品浓度······················································································4.2.2 已知标样浓度测量样品浓度······················································································
5 常见故障排除、仪器维护和检查················································································5.1 常见故障排除···········································································································5.1.1 打开电源开关仪器不动作、屏幕不显示··································································· 165.1.2 仪器自检出错············································································································5.1.3 仪器测量样品的数据不稳定······················································································5.1.4 仪器测量样品的吸光度不准确··················································································5.1.5 打印机不工作或者打印出错······················································································5.2 仪器维护和检查········································································································5.2.1 清扫样品室···············································································································5.2.2 贮藏··························································································································5.2.2.1 完成测量以后············································································································5.2.2.2 长时间不使用············································································································
ii15151515151616161616161616171717
1 概况
1.1 原理
分光光度法分析的原理是利用物质对不同波长光的选择吸收现象来进行物质的定性和定量分析，通过对吸收光谱的分析，判断物质的结构及化学组成。
本仪器是根据相对测量原理工作，即选定某一物质（蒸馏水、空气或试样）作为参比，并设定它的透射比（即透射率T）为100％，而被测试样的透射比是相对于该参比而得到的。透射比（透射率T）的变化和被测物质的浓度有一定函数关系，在一定的范围内，它符合朗伯—比耳定律。
T = I/I。× 100% A = Log (1/T) = KCL
式中：
T-为被测物在给定波长的透射比（透射率）
A-为被测物在给定波长的吸光度值
K-为比消光系数，又称为吸收系数（与入射光波长及被测物质的特性有关） C-为被测物质的浓度
L-为被测物质的厚度（一般与比色皿的厚度有关） I-光透过被测物质后照射到光电转换器上的强度 Io-光透过参比物质后照射到光电转换器上的强度
本仪器就是根据这一原理，结合现代精密光学和最新微电子等高新技术研制而成的分光光度计。
1.2 应用
可供物理学、化学、医学、生物学、药物学、地质学等学科进行科学研究，是广泛应用于化工、药品、生化、冶金、轻工、材料、环保、医学化验等行业中最重要的质量控制仪器之一，是常规实验室必备仪器。
1.3 特点
本仪器具有以下特点：
采用低杂散光，高分分辨率的单光束C-T光路结构单色器，仪器具有低杂散光、高稳定性和测量精度。 采用最新微处理机技术，不仅使仪器具有自动置0％T和100％T、波长自动设置、滤色片自动切换、光源自动切换，同时还具有防止使用者误操作，使用时无后顾之忧。
采用128×64液晶屏显示并中文人机对话，操作简单容易。
仪器还可选配UV-Solution应用软件和计算机连接，进行光谱扫描等功能，使仪器具有更强功能。
1.4 主要技术参数和规格
1.4.1 仪器技术指标
波长范围 200nm～1000nm，可扩展至190nm-1100nm。 波长准确度 ±2nm 波长重复性 ≤1.0nm
透射比测量范围 -1.0～200.0%T 吸光度测量范围 -0.5～3.000A 透射比准确度 ±0.5%T 透射比重复性 ≤0.2%T 杂光 ≤0.5% T 光谱带宽 4nm
数据输出 USB端口、LPT并行打印口
1
紫外可见分光光度计
