YAP mediates the positive regulation of hnRNPK on the lung adenocarcinoma H1299 cell growth

YAP mediates the positive regulation of hnRNPK on the lung adenocarcinoma H1299 cell growth

Lipei Xu;Tingting Zhang;Wensi Huang;Xiaohui Liu;Junlei Lu;Xuejuan Gao;Yun-Fang Zhang;Langxia Liu

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2024(051)007

【摘要】Lung cancer is the leading cause of cancer death worldwide,and non-small cell lung cancer (NSCLC) accounts for 80％-85％ of diagnostic cases.The molecular mechanisms of NSCLC pathogenesis are not well understood.Heterogeneous nuclear ribonucleoprotein K (hnRNPK) is a multifunctional protein that regulates gene expression and signal transduction and closely associated with tumorigenesis,but its mechanism of action in the pathogenesis of NSCLC is unclear.In this study,we observed that the expression pattern of hnRNPK in H1299 lung adenocarcinoma cells varied depending on the cell density in culture.Moreover,hnRNPK stimulated the ability of proliferation and colony formation of H1299 cells,which is important for the multilayered cell growth in culture.We further investigated whether there is an association between hnRNPK and the elements involved in the cell contact inhibition pathway.By using quantitative reverse transcriptase-polymerase chain reaction assay and a YAP activity reporter system,we found that hnRNPK upregulated the mRNA and protein levels and