Construction of the Enhanced Yellow Fluorescent Protein Expression Vector Carrying IFN-γ Gene

Construction of the Enhanced Yellow Fluorescent Protein Expression Vector Carrying IFN-γ Gene

Yuqing Lan;Jian Ge;Yehong Zhuo;Jinlin Wang;Huiyi Chen;Haiquan Liu

【期刊名称】《眼科学报（英文版）》 【年(卷),期】2001(017)003

【摘要】Purpose: To construct the enhanced yellow fluorescent protein (EYFP) vector carryinginterferon-y gene (ifn-γ) in order to provide an ideal reporter in the expression of ifn-γand location of protein in vitro and in vivo.Method: According to the nucleotide sequence of ifn-y gene, a pair of oligonucleotideswas designed as primer whose two end contained nucleotide sequence of EcoR V and NotⅠ restriction endonuclease respectively. The gene encoding for inf-y was amplified usingPCR technique. After the PCR product was retrieved and purified, it was digested withEcoR V and Not Ⅰ restriction endonuclease, and then cloned into the plasmidpIRES-EYFP. The recombinant plasmid plRES-EYFPIFN-γwas identified by restrictionendonuclease enzyme analysis and DNA sequence analysis.Results: The ifn-γ was successfully amplified and verified by partial DNA sequenceanalysis. The recombinant plasmid was correctly screened.Conclusion: The EYFP expression vector carrying ifn-γgene was successfully established.This research work has formed a base for monitoring the ifn-y gene expression andprotein position in living cells.

【总页数】4页(154-157)

【关键词】ifn-y gene;yellow fluorescent protein;cloning, molecular 【作者】Yuqing Lan;Jian Ge;Yehong Zhuo;Jinlin Wang;Huiyi Chen;Haiquan Liu

【作者单位】Sun Yat-Sen Memorial Hospital, Sun Yat-sen University of Medical Science, Guangzhou 510120, China;Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Science, Guangzhou 510060, China;Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Science, Guangzhou 510060, China;Sun Yat-Sen Memorial Hospital, Sun Yat-sen

University

of

Medical

Science,

Guangzhou

510120,

China;Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Science, Guangzhou 510060, China;Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Science, Guangzhou 510060, China 【正文语种】中文 【中图分类】R77 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_eye-science_thesis/0201238871934.html 【相关文献】

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