KIF14在胃癌中的表达及其意义
陈宗营;马恒
【期刊名称】《中国现代普通外科进展》 【年(卷),期】2011(014)012
【摘要】目的:探讨KIF14在胃癌中的表达及其意义.方法:应用实时定量聚合酶链反应技术检测30例胃癌中KIF14 mRNA的表达,应用免疫组织化学方法检测89例胃癌、正常黏膜及35例转移淋巴结中KIF14蛋白的表达.结果:KIF14 mRNA在66.7％的胃癌组织中高表达；KIF14蛋白在胃癌组织中的表达率为62.9％,高于正常黏膜(6.74％,P＜0.05),且与TNM分期(P＜0.01)、浸润深度(P＜0.05)、淋巴结转移(P＜0.05)、远处转移(P＜0.05)及Ki67相关.转移淋巴结中KIF14蛋白表达率(82.9％)高于原发癌和正常黏膜(P＜0.01).KIF14蛋白阳性患者总体生存率和无病生存率均显著降低,KIF14是影响总体生存(P＜0.001)和无病生存(P＜0.001)的独立预后因素.结论:KIF14在胃癌发生发展中起重要作用,可能是新的预后指标.%Objective: To explore the expressiom of KIF14 in gastric cancer and its significance. Methods: KIF14 mRNA expression was detected in 35 cases of gastric cancer and the corresponding para-cancerous tissue by real-time polymerase chain reaction (PCR); KIF14 protein expression was detected by immunohistochemistry on tissue microarray, which containing 89 samples of primary gastric cancer and the corresponding para-cancerous tissue,and 30 paired lymph node metastases (LNM). Reswtot KIF14 mRNA was upregulated more than 2-fold in 66.7% patients with gastric cancer. KIF14 protein was
KIF14在胃癌中的表达及其意义
