番石榴实蝇和瓜实蝇的线粒体L-rRNA基因测序及双翅目
昆虫L-rRNA基因的结构特征与系统发育关系分析
吴培福;熊忠平;徐磊;黄阳洋;潘涌智
【期刊名称】《昆虫学报》 【年(卷),期】2012(055)008
【摘要】The RNA secondary structure contains more information than its primary structure, and can indicate the phylogenetic relationship more really. Therefore, the complete sequences of mitochondrial L-rRNA genes of Bactrocera cucurbitae and B. correcta were sequenced in this study, and the structural model of L-rRNA genes in Diptera was constructed. The characteristics of L-rRNA secondary structure was analyzed, and preliminary phylogenetic features were inferred based on stem-loop parameters and structural sequences of H45 - H47, and structural sequences of L-rRNA genes among 13 families in Diptera. The results showed that the dipterans share the conserved secondary structure of L-rRNA gene; the nucleotide composition and distribution vary within different structure regions of L-rRNA gene; the highest content of completely conserved nucleotides was found in region Ⅳ and Ⅴ ; the region Ⅳ and Ⅴ also contains the highest GC content; the AT-skew is negative in region Ⅳ of L-rRNA gene of the majority of dipteran families; the unique bases within individual family and individually conserved nucleotides between families present higher
番石榴实蝇和瓜实蝇的线粒体L-rRNA基因测序及双翅目昆虫L-rRNA基因的
