Marc-145细胞传代培养条件优化
赵潇;秦钢
【期刊名称】《现代农业科技》 【年(卷),期】2013(000)023
【摘要】Using single test,the effect of recovery temperature serum concentration,growth
solution
pH
value,incubation
temperature,concentration of digestive fluid and passage rate on the subculture for Marc-145 cells was studied.The results showed that the best subculture conditions for Marc-145 cells was when the recovery temperature was 37℃,the serum concentration was 10%,growth solution
pH
value
was
7.2,incubation
temperature
was
37℃,concentration of digestive fluid was 0.25%,and passage rate was 1∶3.%采用单因素法研究在细胞传代培养过程中复苏温度、血清浓度、生长液pH值、培养温度、消化液浓度、传代比例等因素对Marc-145细胞传代培养的影响。结果表明：复苏温度37℃、血清浓度10%、生长液pH值7.2、培养温度37℃、消化液浓度0.25%、传代比例1∶3时Marc-145细胞传代培养效果最佳。
【总页数】3页(270-271,274)
【关键词】Marc-145细胞;传代培养;单因素试验;条件优化 【作者】赵潇;秦钢
【作者单位】浙江经贸职业技术学院，浙江杭州 310018;浙江经贸职业技术学院，浙江杭州 310018
Marc-145细胞传代培养条件优化
