Dantrolene enhances the protective effect of hypothermia on cerebral cortex neurons

Dantrolene enhances the protective effect of hypothermia on cerebral cortex neurons

Sui-yi Xu;Feng-yun Hu;Li-jie Ren;Lei Chen;Zhu-qing Zhou;Xie-jun Zhang;Wei-ping Li

【期刊名称】《中国神经再生研究（英文版）》 【年(卷),期】2015(000)008

【摘要】Therapeutic hypothermia is the most promising non-pharmacological neuroprotective strategy against ischemic injury. However, shivering is the most common adverse reaction. Many studies have shown that dantrolene is neuroprotective inin vitro andin vivo ischemic injury models. In addition to its neuroprotective effect, dantrolene neutralizes the adverse reaction of hypo-thermia. Dantrolene may be an effective adjunctive therapy to enhance the neuroprotection of hypothermia in treating ischemic stroke. Cortical neurons isolated from rat fetuses were exposed to 90 minutes of oxygen-glucose deprivation followed by reoxygenation. Neurons were treated with 40 μM dantrolene, hypothermia (at 33°C), or the combination of both for 12 hours. Re-sults revealed that the combination of dantrolene and hypothermia increased neuronal survival and the mitochondrial membrane potential, and reduced intracellular active oxygen cytoplas-mic histone-associated DNA fragmentation, and apoptosis. Furthermore, improvements in cell morphology were observed. The combined treatment enhanced these responses