FMR1基因敲除小鼠脑组织中Nav1.1的改变及其意义
邸伟;吕桦;刘军;廖卫平;易咏红
【期刊名称】《中华神经医学杂志》 【年(卷),期】2015(014)009
【摘要】目的 探讨Nav1.1在脆性X综合征(FXS)癫痫易感性增加中的可能作用.方法 选用2周龄、4周龄的FVB品系FMR1基因敲除型小鼠(KO2周和KO4周)及同龄野生型小鼠(WT2周和WT4周)为实验对象,应用免疫组化染色方法检测小鼠大脑纹状皮质、颞听皮质、梨状皮质、海马CA1区、CA3区及齿状回中Nav1.1的表达;应用Western blotting检测小鼠大脑皮层和海马组织Nav1.1蛋白含量.结果 免疫组化染色结果显示:KO2周和KO4周小鼠大脑纹状皮质、颞听皮质、梨状皮质、海马CA1区及齿状回区Nav1.1表达的平均吸光度值(2周:0.058±0.006、0.054±0.006、0.130±0.015、0.090±0.009、0.142±0.010;4
周:0.066±0.007、0.060±0.007、0.159±0.018、
0.102±0.015、0.192±0.025)分别较WT2周和WT4周小鼠(2周:0.049±0.007、0.046±0.007、0.118±0.012、0.080±0.009、0.133±0.010;4
周:0.051±0.007、0.048±0.005、0.127±0.012、
0.089±0.012、0.175±0.024)明显增多,差异均有统计学意义(P＜0.05).Western b1otting检测结果显示:KO2周和KO4周小鼠大脑皮层、海马组织Nav1.1蛋白含量(2周:0.635±0.082、0.954±0.111;4周:0.819±0.064、1.145±0.159)分别较WT2周和WT4周小鼠(2周:0.382±0.025、0.555±0.056;4周:0.550±0.040、0.847±0.127)明显增多,差异均有统计学意义(P＜0.05).结论 Nav1.1表达增多可能是引起FXS模型小鼠癫痫易感性增加
的原因之一.%Objective To explore the possible role of Nav 1.1 in the pathogenesis of increased susceptibility to epileptic seizures in FMR1 knockout (FMR1 KO) mice.Methods FVB strain FMR1 KO mice and wild type (WT) controls at ages of 2 and 4 weeks old were chosen;immunohistochemistry was used to determine the expression of Nav
1.1
in
different
brain
regions
(striate
cortex,temporal
cortex,piriform cortex,hippocampus CA1,CA3 and dentate gyrus),and Western blotting was used to determine the Nav1.1 level in the cerebral acustici cortex and hippocampus.Results The mean optical density of Nav1.1 was significantly increased in the striate cortex,temporal acustici cortex,piriform cortex,regions of CA1 and dentate gyrus in FMR1 KO mice at ages of 2 and 4 weeks (2 weeks:0.058±0.006,0.054± 0.006,0.130±0.015,0.090±0.009
and
0.142±0.010;4
and
weeks:0.066±0.007,0.060±0.007,0.159±0.018,0.102±0.015
0.192±0.025) as compared with the age-matched WT mice (2 weeks:0.049±0.007,0.046±0.007,0.118±0.012,0.080±0.009 0.133±0.010;4
and
weeks:0.051±0.007,0.048±0.005,0.127±
0.012,0.089±0.012 and 0.175±0.024,P＜0.05).The levels of Nav1.1 in the cerebra1 cortex and hippocampus in FMR1 KO mice at ages of 2 and 4 weeks (2 weeks:0.635±0.082 and 0.954±0.111;4 weeks:0.819 ±0.064 and 1.145 ±0.159) were also significantly increased as compared with the age-matched WT mice (2 weeks:0.382±0.025 and 0.555±0.056;4
weeks:0.550±0.040 and 0.847±0.127,P＜0.05).Conclusion Increased expression of Nav1.1 might play an important role in the increased susceptibility to epileptic seizures in FMR1 KO mice. 【总页数】5页(894-898)
【关键词】脆性X综合征;Nav1.1;癫痫;易感性 【作者】邸伟;吕桦;刘军;廖卫平;易咏红
【作者单位】710001西安,陕西省人民医院神经内科;710001西安,陕西省人民医院神经内科;710001西安,陕西省人民医院神经内科;510260广州,广州医科大学第二附属医院神经内科,神经科学研究所;510260广州,广州医科大学第二附属医院神经内科,神经科学研究所 【正文语种】中文 【中图分类】R742.1 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-neuromedicine_thesis/0201237779796.html 【相关文献】
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FMR1基因敲除小鼠脑组织中Nav1.1的改变及其意义
