Inhibition of quorum sensing in Chromobacterium violaceum by Syzygium cumini L.and Pimenta dioica L.

Inhibition of quorum sensing in Chromobacterium violaceum by Syzygium cumini L.and Pimenta

dioica L.

Halkare Suryanarayana Vasavi; Ananthapadmanabha Bhagwath Arun; Punchapady Devasya Rekha

【期刊名称】《《亚太热带生物医学杂志：英文版》》 【年(卷),期】2013(000)012

【摘要】Objective:To investigated into the anti-quorum sensing（QS）activity of Syzygium cumini L.（S.cumini）and Pimenta dioica L.（P.dioica）using Chromobacterium violaceum（C.violaceum）strains.Methods:In this study,anti-QS activity of ethanol extract of Syzygium cumini L.and Pimenta dioica L.were screened using C.violaceum CV026 biosensor bioassay.By bioassay guided fractionation of 5.cumini and P.dioica,ethyl acetate fraction（EAF）with strong anti-QS activity was separated.Inhibition of QS regulated violacein production in C.violaceum ATCC12472 by EAF was assessed at different concentrations.The effect of EAF on the synthesis of autoinducer like N-acyl homoserine lactone（AHL）was studied in C.violaceum ATCC31532 using its mutant C.violaceum CV026 by standard methods.Results:EAF inhibited violacein production in C.violaceum ATCC12472 in a concentration dependent manner without significant reduction in bacterial growth.Complete inhibition of violacein production was