骨髓间充质干细胞与复合I型胶原和骨形态发生蛋白2的聚
乳酸乙醇酸构建组织工程骨
查振刚;李劼若;周长忍;姚平;吴昊;刘宁;黄春华;杨淑野;王双利
【期刊名称】《中华生物医学工程杂志》 【年(卷),期】2008(014)003
【摘要】Objective To construct the tissue engineering bone by bone marrow mesenchymal stem cells (BMSCs) cultured on poly-lactide-co-glycolic acid (PLGA) modified with type Ⅰcollagen and recombinant human bone morphagenetic protein 2 (rhBMP-2). Methods BMSCs were isolated and cultured in vitro. The cellular configuration was observed continually under inverted phase-contrast microscope, and the cell exterior antigen was analyzed by flow cytometry method. PLGA was made by phase separation and modified with type Ⅰ collagen and rhBMP-2. The biomaterial structure was examined by scan electron microscope. The third generation BMSCs were cultured on PLGA. The adhesion of cell to biomaterial was examined by scan electron microscope as well. The complex of BMSCs-PLGA was then cultured for 6 h in SD rat muscle and was observed two months later by HE staining. Results BMSCs could be isolated and cultured in vitro, and the cell morphology was spindle. They expressed CD29, CD44, but did not express CD34,CD45. The average pore diameter of PLGA modified with type Ⅰ collagen and rhBMP-2 was 100 μm, the interval porosity was
骨髓间充质干细胞与复合I型胶原和骨形态发生蛋白2的聚乳酸乙醇酸
