TGF-β1短时间干预间充质干细胞可增加其CXCR4的表达
及迁移能力
禹虔;杨述华;冯勇;刘先哲;陈东;王小红;陈超
【期刊名称】《华中科技大学学报（医学版）》 【年(卷),期】2012(041)005
【摘要】Objective To investigate the effects of short-term exposure of mesenchymal stem cells(MSCs) to transforming growth factor (β1 (TGF-β1 ) on the expression of chemokine receptor 4 (CXCR4) and the migratory capacity of the cells in vitro. Methods MSCs were intervened with different concentrations of TGF-(3i. MSCs were identified by flow cytometry. The expression of CXCR4 was detected by using Western blot and real-time quantitative PCR. The migratory ability of MSCs was measured by cell migration assay. The methylation status of CXCR4 gene in MSCs was examined by methylation-specific PCR (MSP). Results Short-term exposure of MSCs to TGF-pi resulted in increased CXCR4 protein expression in a concentration-and time-dependent manner. The CXCR4 protein expression peaked at the time of 24 h. Meanwhile, CXCR4 mRNA level was increased in a concentration-dependent way at 24 h after exposure of MSCs to TGF-β1 and migration ability of MSCs was enhanced, though methylation status of CXCR4 gene in MSCs was not modified after TGF-β1 treatment. Conclusion After the MSCs were treated by TGF-|3i ,the expression of CXCR4 was increased and the
migration ability of MSCs was enhanced.%目的 观察转化生长因子β1 (TGF-β1)短时间干预间充质干细胞(MSCs)后趋化因子受体4(CXCR4)的表达及对MSCs迁移能力的影响.方法 MSCs被不同浓度(0、0.01、0.1、1、10 ng/mL)和时间梯度(6、12、24、48 h)的TGF-β1干预后,采用流式细胞术鉴定MSCs细胞,通过Western blot和real-time quantitative PCR检测CXCR4的表达,并使用transwell小室实验来鉴定MSCs细胞的迁移能力.CXCR4基因的甲基化状态通过甲基化特异性PCR(methylation-specific PCR,MSP)检测.结果 将MSCs细胞短时间暴露在TGF-β1中会导致CXCR4的蛋白水平以浓度依赖性的方式升高,且随着干预时间的延长表达升高,于24 h时达到高峰；而此时,检测MSCs细胞暴露于TGF-β124 h这个时间点的CXCR4的mRNA水平,发现其以浓度依赖性的方式升高,而且MSCs的迁移能力增强,但MSP的结果显示CXCR4基因甲基化状态并未发生改变.结论 MSCs细胞经TGF-β1干预后,其CXCR4的表达增加,迁移能力增强. 【总页数】6页(523-528)
【关键词】间充质干细胞;趋化因子受体4;转化生长因子β1 【作者】禹虔;杨述华;冯勇;刘先哲;陈东;王小红;陈超
【作者单位】华中科技大学同济医学院附属协和医院骨科,武汉430022;华中科技大学同济医学院附属协和医院骨科,武汉430022;华中科技大学同济医学院附属协和医院骨科,武汉430022;华中科技大学同济医学院附属协和医院骨科,武汉430022;华中科技大学同济医学院附属协和医院骨科,武汉430022;华中科技大学同济医学院附属协和医院骨科,武汉430022;华中科技大学同济医学院附属协和医院骨科,武汉430022
【正文语种】中文 【中图分类】R349.6 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_acta-medicinae-universitatis-scientiae-technologiae-huazhong_thesis/0201245914649.html 【相关文献】
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TGF-β1短时间干预间充质干细胞可增加其CXCR4的表达及迁移能力
