The influence of physiological environment on the targeting effect of aptamer-guided gold

The influence of physiological environment on the

targeting effect of aptamer-guided gold

nanoparticles

Ding Ding;Yinling Zhang;Edward A.Sykes;Long Chen;Zhuo Chen;Weihong Tan

【期刊名称】《纳米研究（英文版）》 【年(卷),期】2024(012)001

【摘要】Aptamer guided nanomedicine shows great promise in targeted cancer therapies.However the loss of targeting capacity during in vivo or clinical trials has largely hindered its popularity and there are no systematic studies to elucidate the causes.Herein,we investigated such loss of targeting capacity by examining how the physiological milieu

affected

targeting

effect.Aptamer

functionalized

gold

nanoparticle (AuNP)was chosen as the model and exposed to human blood serum that is used to mimic physiological milieu.Dynamic light scattering (DLS),flow cytometry and label-free liquid chromatography tandem mass spectrometry (LC-MS/MS) were employed to determine variations of NPs' surface chemistry and biological identities changes after serum exposure.Results showed that the targeting ability loss was caused by protein corona blocking,replacement and enzymatic cleavage of surface aptamer targeting ligands.Noteworthy,the aggregation issue is critical for the smaller NPs.Analysis of the protein corona profile indicated the accumulation of immune-related proteins on the surface

of aptamer-conjugated NPs,which could induce immune

response,resulting in rapid clearance of NPs. 【总页数】7页(129-135) 【关键词】

【作者】Ding Ding;Yinling Zhang;Edward A.Sykes;Long Chen;Zhuo Chen;Weihong Tan

【作者单位】Molecular Science and Biomedicine Laboratory(MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering and College of Life Sciences, Hunan University, Changsha 410082, China;Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine,

Shanghai

Jiao

Tong

University,Shanghai

200240,

China;Molecular Science and Biomedicine Laboratory(MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering and College of Life Sciences, Hunan University, Changsha 410082, China;School of Medicine, Queen's University, 81 Barrie Street, Kingston, ON, K7L 3N6, Canada;Faculty of Science and Technology, University of Macau, Avenida da Universidade, Macau Taipa 999078, China;Molecular Science and Biomedicine Laboratory(MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering and College of Life Sciences, Hunan University, Changsha 410082,

China;Molecular Science and Biomedicine Laboratory(MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering and College of Life Sciences, Hunan University, Changsha 410082, China;Department of Chemistry, Departments of Physiology and Functional Genomics, Center for Research at the Bio/Nano Interface, UF Health Cancer Center,UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611, USA 【正文语种】英文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_nano-research-english_thesis/0201271732658.html 【相关文献】

1.The influence of physiological environment on the targeting effect of aptamer-guided gold nanoparticles [J], Ding Ding[1,5]; Yinling Zhang[1]; Edward A.Sykes[2]; Long Chen[3]; Zhuo Chen[1]; Weihong Tan[1,4] 2.Targeting orthotopic gliomas with renal-clearable luminescent gold nanoparticles [J], Chuanqi Peng[1] Xiaofei Gao[2] Jing Xu[1] Bujie Du[1] Xuhui Ning[1] Shaoheng Tang[1] Robert M. Bachoo[3] Mengxiao Yu[1] Woo-Ping Ge[2] Jie Zheng[1]

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of

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