7000型支持两种定量化学：TaqMan荧光探针和SYBR Green I荧光染料。其熔解曲线(Dissociation Curve)功能用于判断PCR扩增反应是否特异，有无杂带生成。
进一步阅读材料基本方法[1] Lie, Y. S. and C. J., Petropoulos. \′nuclease assays.\Orlando, C., P., Pinzani, and M., Pazzagli. \quantitative PCR.\绝对定量[3] Becker K., D. Pan and C.B. Whitley. 1999. Real-time quantitative polymerase chain reaction to assess gene transfer. Hum. Gene Ther. 10: 2559-2566, 1999.[4] de Kok, J.B., Hendriks, J.C., van Solinge, W.W., Willems, H.L., Mensink, E.J., and Swinkels, D.W. Use of real-time quantitative PCR to compare DNA isolation methods. Clin.Chem. 44(10):
2201-2204, 1998. [5] Wang X., X. Li, R.W. Currie, R.N. Willette, F.C. Barone and G.Z. Feuerstein. Application of real-time polymerase chain reaction to quantitate induced expression of interleukin-1beta mRNA in ischemic brain tolerance. J. Neurosci. Res. 59(2): 238-46,2000.相对定量[6] de Kok, J.B.,
Ruers, T.J., van Muijen, G.N., van Bokhoven, A., Willems, H.L., and Swinkels, D.W. Real -time quantification of human telomerase reverse transcriptase mRNA in tumors and healthy tissues. Clin.Chem. 46(3): 313-8,2000.[7] Moody, A., Sellers, S., and Bumstead, N. Measuring infectious bursal disease virus RNA in blood by multiplex real-time quantitative RT-PCR. Virol. Methods 85(1-2) 55-64,2000.[8] Zhang, A.W., Hartman, G.L., Curio-Penny, B., Pedersen, W.L., and Becker, K.B. Molecular Detection of Diaporthe phaseolorum and Phomopsis longicolla from Soybean Seeds. Phytopathology 89(9): 796-804, 1999.
实时荧光定量PCR原理和实验
