Predicting Polymerase Ⅱ Core Promoters by Cooperating Transcription Factor Binding Sites

Predicting Polymerase Ⅱ Core Promoters by Cooperating Transcription Factor Binding Sites in

Eukaryotic Genes

Xiao-Tu MA;Min-Ping QIAN;Hai-Xu TANG

【期刊名称】《生物化学与生物物理学报：英文版》 【年(卷),期】2004(036)004

【摘要】Several discriminate functions for predicting core promoters that based on the potential cooperation between transcription factor binding sites (TFBSs) are discussed. It is demonstrated that the promoter predicting accuracy is improved when the cooperation among TFBSs is taken into consideration.The core promoter region of a newly discovered gene CKLFSF1 is predicted to locate more than 1.5 kb far away from the 5′ end of the transcript and in the last intron of its upstream gene, which is experimentally confirmed later. The core promoters of 3402 human RefSeq sequences, obtained by extending the mRNAs in human genome sequences, are predicted by our algorithm, and there are about 60% of the predicted core promoters locating within the ± 500 bp region relative to the annotated transcription start site. 【总页数】9页(250-258)

【关键词】promoter;transcription factor binding site;transcription start site;gene finding