甘薯中咖啡酰奎尼酸类化合物的HPLC法比较分析
秦玉芝;付鹏飞;全华;熊兴耀;陆英
【期刊名称】《农业科学与技术（英文版）》 【年(卷),期】2016(017)012
【摘要】Objective] An HPLC method for the determination of caffeoylquinic acid compounds in sweet potato was developed in the present research. Quantitative analysis was performed on 11 sweet potato genotypes to provide a reference for the development and utilization of sweet potato, especial y on the aboveground part. [Method] Caffeoylquinic acid compounds were extracted from sweet potato leaves by ultrasonic treatment for 20 min with 200 volumes of methanol. The mobile phase of HPLC was acetonitrile -0.2% aqueous phosphoric acid. Chlorogenic acid, 4, 5-O-coffeocylquinic acid, 3, 5-O-coffeeacylquinic acid and 3, 4-O-coffeeacylquinic acid were separated wel by gradient elution, with recovery rate of 94.74%, 102.63%, 100.01% and 101.05%, respectively. [Result] The contents of caffeoylquinic acids were significantly different between sweet potato genotypes and tissue parts. Among these compounds, the content of bi-caffeoylquinic acids was the highest in the aboveground part, and the content of chlorogenic acid was the highest in the root tubers. The content of caffeoylquinic acid in different parts of the plant was: 9.77-40.86 mg/g in stem apex, 2.68-13.97 mg/g in mature leaves, 0.56-7.90 mg/g in
甘薯中咖啡酰奎尼酸类化合物的HPLC法比较分析
