In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L.

In vitro anti-diabetic, anti-obesity and antioxidant proprieties of Juniperus phoenicea L. leaves from

Tunisia

佚名

【期刊名称】《亚太热带生物医学杂志（英文版）》 【年(卷),期】2014(000)0z2

【摘要】Objective: To examine chemical composition and antioxidant activity as well as the in vitroα-amylase and pancreatic lipase inhibitory activities of the essential oil and various extracts of Juniperus phoenicea (J. phoenicea).
Methods: Essential oil obtained by steam distillation were analyzed by gas chromatography-mass spectrometry technique. The antioxidant activity of the essential oil and various extracts of J. phoenicea were determined by DPPH andβ-carotene bleaching

methods.

Results:

Gas

chromatography-mass

spectrometry analysis of the J. phoenicea essential oil resulted in the identification of 37 compounds, representing 96.98% of the oil; α-Pinene (24.02%), limonene (7.94%), D-3-Carene (16.9%), Germacrene D (11.98%), Germacrene B (5.40%) and δ-cadinene (6.52%) were the major compounds. The IC50 values of essential oil, hexane and methanol extracts against α-amylase were 35.44, 30.15 and 53.76 μg/mL respectively, and those against pancreatic lipase were 66.15, 68.47 and 60.22 μg/mL respectively, suggesting powerful anti-diabetic and anti-