High prevalence ofblaCTX-M inEnterobacteriaceae isolates from the Kingdom of Bahrain

High prevalence ofblaCTX-M inEnterobacteriaceae

isolates from the Kingdom of Bahrain

Khalid M Bindayna;Mariam Murtadha

【期刊名称】《亚太热带医药杂志（英文版）》 【年(卷),期】2011(000)012

【摘要】Objective:To determine the molecular epidemiology of extended-spectrum β-lactamase (ESBL) by testing a cohort of clinicalESBL-producing bacterial isolates that were isolated in the Kingdom of Bahrain.Methods:ESBLproducingEnterobacteriaceae isolates (based on phenotypic tests) were collected from Microbiology Laboratory of the Salmaniya Medical Complex, Bahrain between January-June2006. Antibiotic susceptibility to a panel of antibiotics was performed andblaCTX-M genes were detected by multiplexPCR.Results: A

total

of230

isolates

(Escherichia

coli,n=180;Klebsiella

pneumoniae,n=50) were studied,98% were CTX-M type. ForEscherichia coli isolates,65 (36.1%)harboredCTXM+TEMcombination and68(37.8%) had CTX-M alone. In contrast, forKlebsiella pneumoniae isolates only 5 (10.0%) harbored the CTX-M combination, and none had CTX-M only. The blaCTX-Mgene was found predominantly in urine isolates (n=145/230; 63.0%). Sensitivity to imipenem and nitrofurantoin was100% and 60%, respectively.CTX-M carriage was associated with the resistance to

fluoroquinolones,

trimethoprim-sulfamethoxazole

and