碲化镉量子点对大鼠肝脏细胞色素P450酶含量的影响

碲化镉量子点对大鼠肝脏细胞色素P450酶含量的影响

李佳宜;黄沛力

【期刊名称】《中华劳动卫生职业病杂志》 【年(卷),期】2024(036)003

【摘要】Objective The effect and mechanism of cadmium telluride quantum dots (CdTe QDs) on cytochrome P450 (CYP450) in the liver of rat were investigated.Methods CdTe QDs (Ex 350 nm,Em 600 nm) were incubated with microsomes in final concentrations (0.5,5,50 μmol/L) using rat liver.And the content of CYP450 was determined by mixed incubation system as time(15,30,45 min) went on.Relationship also was detected between particle sizes(Em 620,580,540 nm;CdTe QDs-2,CdTe QDs-3,CdTe QDs-4) and expression of CYP450.Twenty male Wistar rats were

randomly

divided

into

exposed

groups

at

various

concentrations(0.25,2.5 and 12.5 μmol/kg) of CdTe QDs via tail vein injection,the control group was injected with PBS.Results In vitro,CdTe QDs(0.5,5,50 μmol/L) could significantly increase the content of CYP450 in rat liver microsomes(P＜0.05),which increased first and then decreased with the dose adding.Moreover,the trend along with the exposure time (15,30,45 min) was the same as that in dosages at certain concentration (P＜0.01).For different particle sizes,the smaller CdTe QDs were,the higher content increased,the content of CYP450 in group CdTe QDs-4 was the highest(P＜0.05).In vivo,experiment proved that CdTe