中国人群中肾上腺素α2A受体基因SNP的分布及其对基
因表达的影响
袁栎;沈士弼;罗超权
【期刊名称】《生物医学研究杂志（英文版）》 【年(卷),期】2003(017)006
【摘要】Objective: To scan single nucleotide polymorphism ( SNP ) in Chinese alpha-2A adrenergic receptor( α2A-AR )gene and study the effcts of the SNP on the gene expression .Methods: The complete sequence of α2A-AR gene was analyzed with autonated DNA sequencer to scan SNPs. Genomic DNA was extracted from whole blood and a 239 bp fragmnent containing the G/C polymorphism was amplified with PCR using a pair of specific primers.PCR-RFLP was used to perform the genotyping of the SNP at the site- 1 296 bp of the people in the North of China. Electrophoresis mobility shift assay( EMSA )was used to study the binding of the 390bp fragments(- 1 414-1 025 bp) with G or C at the site-1 296 bp and nuclear extracts. Results: In our study, two SNPs werefound in α2A-AR gene. Allele frequeneies of the SNP at the site- 1 296 bp were 0.61 and 0.39 for G and C, and the genotype frequencies were 0.34,0.54 and 0.13 for GG, GC and CC respectively from the people in the North of China.In the EMSA, a specific binding appeared in the complex of nuclear extracts and DNA with C at- 1 296 bp. Conclusion: Two SNPs exist in a2A-AR gene from the people in the North of China,
中国人群中肾上腺素α2A受体基因SNP的分布及其对基因表达的影响
