AMD3100抑制胃癌细胞的增殖和侵袭能力及其机制研究
刘丽娟;关国锋;王小娟;薛琳;李亚妮;王飙落;梁树辉;丁杰
【期刊名称】《现代肿瘤医学》 【年(卷),期】2015(000)001
【摘要】Objective:To explore the effect of AMD3100,which is the specific inhibitor of CXCR4,on proliferation and invasion of gastric cancer cells and its probable mechanism. Methods:Western blot was used to detect the expres-sion of CXC chemokine receptor-4(CXCR4)in different metastatic potential cell lines. AMD3100 at different final concentrations was applied to treat with human gastric cancer cell line MKN28-M and MKN28-NM. CCK-8 assay was used to detect their proliferation. Transwell assay was used to identify the invasive ability in MKN28 -M and MKN28-NM cell lines. Western blot was employed to detect the expressions of matrix metalloproteinase-9( MMP-9)and vascular endothelial growth factor(VEGF)in MKN28-M cell line after incubation with AMD3100. Results:Western blot showed that expression level of CXCR4 in MKN28-M cells was higher than MKN28-NM,while with hardly expression in MKN28 cells. The proliferation of MKN28-M and MKN28-NM were significantly suppressed by AMD3100 in a dose-dependent
manner(p<0.
05).
AMD3100(100
and
1000ng/ml)significantly inhibited the invasion ability of MKN28-M and MKN28-NM cells(p<0. 01). Treatment with AMD3100 markedly reduced
AMD3100抑制胃癌细胞的增殖和侵袭能力及其机制研究
