Interferon as a Mucosal Adjuvant for an Influenza Vaccine in Pigs

Interferon as a Mucosal Adjuvant for an Influenza

Vaccine in Pigs

Lirong Liu[1,2];Wenhui Fan[1];He Zhang[1];Shuang Zhang[1];Liang Cui[1,2];Meng Wang[1,2];Xiaoyuan Bai[1,2];Wenxian Yang[1,2];Lei Sun[1,2];Limin Yang[1];Wenjun Liu[1,2];Jing Li[1,2];;;;;;;;;

【期刊名称】《中国病毒学：英文版》 【年(卷),期】2019(034)003

【摘要】Interferon,a natural protein that is produced by a variety of cells during viral infection,activates the transcription of multiple functional genes in cells,regulates synergy among various signaling pathways,and mediates many biological functions such as antiviral activity,immune regulation,and cell growth.However,clinical research on interferon in livestock

is

lacking.In

this

study,recombinant

porcine

interferon(PoIFNα)was used as an adjuvant,in combination with inactivated influenza virus,to vaccinate 6-week-old pigs via nasal infusion.The transcription of target genes was then monitored and the functions of PoIFNαwere determined with respect to the activation of mucosal immunity.We found that a combination of low-dose PoIFNαand in activated influenza virus could significantly up-regulate the expression of immunoregulatory cytokines such as IL-2,IL-1β,IFN-γ,IL-6,and IL-10 by real-time PCR,suggesting the induction of a strong mucosal innate immune response after administration.In addition,low-