食管鳞癌外周血循环肿瘤细胞与临床病理特征的关系
陈威鹏;李印;袁东风;李浩淼;袁俊;秦建军
【期刊名称】《中华实验外科杂志》 【年(卷),期】2019(036)003
【摘要】目的 探讨表达上皮-间充质转化(EMT)标志物的外周血循环肿瘤细胞(CTCs)对食管鳞癌(ESCC)的诊断价值及其与ESCC临床病理特征和治疗效果的相关性.方法 纳入174例首诊ESCC患者为观察对象,40例健康志愿者为阴性对照,根据ESCC不同治疗阶段及临床分期对其CTCs进行定性、定量检测分析.结果 (1)174例ESCC患者外周血总CTCs中位数显著高于40例健康志愿者(21比0),阳性率也显著高于健康志愿者(99.4％比35.0％),上皮型CTCs、间质型CTCs与总CTCs检测在受试者工作特征曲线(ROC)下面积分别为0.813、0.782、0.991,其最佳诊断界点分别为2、0、3,总CTCs对肿瘤的诊断效能最高,敏感性可达90％以上.(2)ESCC患者中临床分期越晚,其间质型CTCs计数越多(P＜0.01).(3)39例给予2周期新辅助化疗的ESCC患者,依据RECIST1.1将其分为:完全缓解(CR)、部分缓解(PR)、稳定(SD)、进展(PD),分别为2、20、13、4例.2周期化疗后间质型CTCs阳性率在PD/SD组(70.6％)明显高于CR/PR组(36.4％),P＜0.05.(4)对于直接手术治疗的47例ESCC患者,术前术后血样检测结果分析表明总CTCs计数在术后明显下降(P＜0.01),而间质型CTCs则呈现上升趋势(P＜0.01).结论 间质型CTCs作为ESCC的一项生化检测指标,具有一定的诊断价值,其与临床分期及化疗效果具有一定的相关性.%Objective To evaluate the diagnostic value of circulating tumor cells (CTCs) with ex pressing epithelial-mesenchymal transitions (EMT) biomarkers in
esophageal squamous cell carcinoma (ESCC) patients and investigated the association with their clinicopathological parameters.Methods We included 174 patients with ESCC as the observation group and 40 healthy volunteers as negative control group.According to the different treatment stages,blood samples from ESCC patients were analyzed qualitatively and quantitatively for CTCs in peripheral blood.Results (1) The total CTCs in 174 ESCC patients was higher than that in 40 healthy volunteers (21 vs.0),and the positive rate was also higher than that of healthy volunteers (99.4％ vs.35.0％),the area under receiver operating characteristic curve (ROC) of epithelial CTCs,mesenchymal CTCs and total CTCs were 0.813,0.782 and 0.991,respectively.The clinical diagnostic critical point of each phenotypes of CTCs were 2,0 and 3,respectively;(2) For ESCC patients,the more the mesenchymal CTCs,the later the clinical stage;(3) Follow-up blood specimens were obtained from neoadjuvant chemotherapy in ESCC patients.Complete response (CR),partial response (PR),stable disease (SD) and progressive disease (PD)
after
treatment
were
seen
in
2,20,13
and
4
patients,respectively.Patients with a negative mesenchymal CTCs status after two cycles of chemotherapy had a good chemosensitivity (P ＜ 0.05),and the positive rate of mesenchymal CTCs in SD/ PD (70.6％) was significantly higher than that in CR/PR (36.4％) group;(4) We compare the changes in CTCs between preoperative and postoperative of 47
ESCC patients,and the analysis revealed that the total CTCs of postoperative
patients
were
decreasing
(P
＜
0.01),however,mesenchymal CTCs were increasing dramatically (P ＜ 0.01).Conclusion Mesenchymal CTCs as a biochemical marker of ESCC has a certain value in diagnosis of ESCC,which is significantly correlated with the clinical stage and chemosensi tivity of the tumor. 【总页数】4页(546-549)
【关键词】食管癌;循环肿瘤细胞;上皮-间充质转化;波形蛋白 【作者】陈威鹏;李印;袁东风;李浩淼;袁俊;秦建军
【作者单位】河南科技大学附属三门峡市中心医院胸外科 472000;国家癌症中心中国医学科学院北京协和医学院肿瘤医院胸外科,北京 100730;郑州大学附属肿瘤医院胸外科 450008;郑州大学附属肿瘤医院胸外科 450008;郑州大学附属肿瘤医院胸外科 450008;国家癌症中心中国医学科学院北京协和医学院肿瘤医院胸外科,北京 100730 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chinese-journal-experimental-surgery_thesis/0201270478771.html 【相关文献】
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食管鳞癌外周血循环肿瘤细胞与临床病理特征的关系
