脑源性神经营养因子对苯丙氨酸诱导的Cdc42、Rac1和
RhoA蛋白表达的影响
张拥军;袁小兵;顾学范
【期刊名称】《临床儿科杂志》 【年(卷),期】2009(027)011
【摘要】Objective To explore whether phenylalanine affect Cdc42, Racl, and RhoA expression and disturb dendritic development. To determine the effects of brain-derived neurotrophic factor (BDNF) on this process. Methods Neurons were cultivated up to 3 days and then treated with 0.9 mmol/L phenylalanine or 100 ng/ml BDNF. Dendritic number were determined by morphologic analysis. Cdc42, Racl, and RhoA protein expression were examined by Western blotting analysis. Results The number of dendrites in cultured neurons reduced two days after being treated with phenylalanine, while BDNF could rescue this change (P < 0.01), furthermore, BDNF was found to inhibit phenylalanineinduced down-regulation of Cdc42, Racl, and RhoA protein expression (P < 0.01). Conclusions Our study indicated that the protective effect of BDNF against phenylalanine-induced neuronal injury is probably mediated by expression of Cdc42, Racl, and RhoA. It suggested a potential neuroprotective action of BDNF in prevention and treatment of brain injury in the patients with phenylketonuria.%目的 观察脑源性神经营养因子(BDNF)对苯丙氨酸诱导的神经元树突的生长及其Cdc42、Rac1和
脑源性神经营养因子对苯丙氨酸诱导的Cdc42、Rac1和RhoA蛋白表达的影响
