Biocompatibility of iron carbide and detection of metals ions signaling proteomic analysis

Biocompatibility of iron carbide and detection of metals ions signaling proteomic analysis via

HPLC/ESI-Orbitrap

Murtaza Hasan;Wenlong Yang;Yanmin Ju;Xin Chu;Yun Wang;Yulin Deng;Nasir Mahmood;Yanglong Hou

【期刊名称】《纳米研究（英文版）》 【年(卷),期】2017(010)006

【摘要】Recently,magnetic nanoparticles (NPs) have been extensively used in food industry and biomedical treatments.However,the biocompatibility

mechanism

on

expression

proteomics,before

consideration of magnetic NPs for clinical application,has not yet been fully elucidated.Therefore,this study was undertaken to identify potential biomarkers of metal ion signaling proteins in human cervical cancer cell line (HeLa) cells.Here,we report the in vitro investigations of the cell cycle response and significant changes in protein abundance of HeLa cells when exposed to self-tailored hydrophilic Fe2C NPs.The comparative proteomic approach based on 18O labeling coupled with high performance liquid chromatography/electrospray ionization with ion trap mass analyzer (HPLC/ESI-Orbitrap) was applied,and 394 proteins were identified.There were 46 significantly differentiated proteins based on the specific metal ion signaling response.Among them,60S ribosomal protein L37a,serine/arginine-rich splicing factor