HPLC法测定贞芪扶正薄膜衣片中齐墩果酸的含量
梁琪;马君义;袁才英
【期刊名称】《中国医药指南》 【年(卷),期】2012(000)025
【摘要】 Objective To determine the contents of the Zhenqifuzheng film-coatd
tables
by
HPLC.
Methods The HPLC method was
established for the content determination of oleanolic acid in the Zhenqifuzheng film-coatd tables,was used with a mobile phase consisting
of
acetonitrile-methanol-water-ammonium
acetate(70:16:14:0.5) ,the flow rare was 1.0 mL/min,the detection wavelength was 215 nm,and the column tempreture was room temperature. Results A good linearity was obtained in the range of 0.00824~2.06mg/mL
(r=0.9999)
,
linear
equation
is
y=9905.62+2012.30x ,the average recovery was 99.57% with RSD of 1.01%(n=5). Conclution
The
established
method
is
simple,fast,reliable,accurate, and can be used as quality control of the Zhenqifuzheng film-coatd tables.% 目的 建立贞芪扶正薄膜衣片的含量测定方法.方法 采用高效液相色谱法测定贞芪扶正薄膜衣片中齐墩果酸的含量.流动相：乙腈-甲醇-水-乙酸铵(70∶16∶14∶0.5)；流速为1.0mL/min,检测波长：215nm；柱温为室温.结果 在0.00824~2.06mg/mL范围内线性关系良好(r=0.9999),线性方程为
y=9905.62+2012.30x.平均回收率为
99.57%,RSD=1.01%(n=5).结论 所建立的方法简便、快速,结果可靠、准确,可
HPLC法测定贞芪扶正薄膜衣片中齐墩果酸的含量
