雷帕霉素对蛛网膜下腔出血大鼠早期脑损伤的保护作用
马仁政;曹守明;赛因巴雅尔;李建民
【期刊名称】《脑与神经疾病杂志》 【年(卷),期】2024(026)002
【摘要】目的 探讨雷帕霉素(RAP)对蛛网膜下腔出血(SAH)模型大鼠早期脑损伤的保护作用.方法 80只雄性SD大鼠随机分成假手术(Sham)组、SAH组、低、高剂量(RAP,50ug·kg-1·d-1和100ug·kg-1·d-1)组.采用枕大池二次注血法制作SAH大鼠模型;TUNEL法检测皮质区凋亡细胞、巴比妥酸法检测大脑皮质组织中丙二醛(MDA)水平、酶联免疫吸附测定法(ELISA)检测白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α).结果 与Sham组比较,SAH组皮质区凋亡神经细胞数量、MDA含量、IL-1β和TNF-α水平增加(P<0.05);与SAH组比较,RAP组凋亡神经细胞数量、MDA含量、IL-1β和TNF-α水平降低;高剂量RAP组更为显著(P<0.05).结论 RAP对SAH具有神经保护作用,可降低SAH后氧化应激和炎症反应程度,减少神经细胞凋亡.%Objective To investigate the protecitive effects of rapamycin (RAP)on early brain injury after subarachnoid hemorrhage(SAH)in rats. Method Eighty male Sprague-Dawley rats were randomly divided into four groups: sham operated(Sham) group, SAH group, low dose and high dose RAP group. The SAH model was established by using the two injection of the classic pillow. The quantities of apoptotic cells in cortex was measured by TUNEL method; The malondialdehyde (MDA) level in cortex was detected with thiobarbituric acid; and interleukin-1 beta (IL-1β)and tumor necrosis
雷帕霉素对蛛网膜下腔出血大鼠早期脑损伤的保护作用
