自噬在A549肺腺癌细胞耐厄洛替尼中的作用研究
徐睿;孙建国;陈正堂
【期刊名称】《重庆医学》 【年(卷),期】2013(042)012
【摘要】Objective To investigate the effect of autophagy in A549's resistance of erlotinib. Methods After A549 cells were treated with different levels of erlotinib,autophagy was determined by cell immunofluorescence and protein lever of LC3 Ⅱ was de termined by Western blot at certain times. And then afer inhibiting autophagy by the pharmacological inhibitor 3 methyladenine(3 MA) ,the cell cycle and apoptosis rate were determined by flow cytometry(FCM). Results Compared to control group (0. 1%DM SO) ,the protein levers of MAP1 LC3 Ⅱ in both A549 and A549/ER were upregulated at low levels(l,5 μmol/L). The protein le ver of MAP1 LC3 Ⅱ in A549/ER was still upregulated while the protein lever of LC3 Ⅱ in A549 was downregulated at the high level(25 μmol/L). Moreover,A549/ER's apoptosis induced by erlotinib was enhanced after inhibiting autophagy by 3 MA,the ap optosis rate rose from 7. 76% to 18. 85 %. Conclusion Autophagy thus represents a role in A549/ER resistance of erlotinib TKIs and inhibiting autophagy may be an approach to improve the efficacy of erlotinib.%目的 探讨自噬在A549肺腺癌细胞耐厄洛替尼中的作用.方法 不同水平厄洛替尼处理A549细胞及其获得性耐厄洛替尼细胞(A549/ER)后,细胞
免疫荧光和Western blot观察自噬标记蛋白微血管相关蛋白1轻链3-Ⅱ(MAP1-LC3-Ⅱ)的变化;应用自噬抑制剂3-甲基腺嘌呤(3-MA)抑制自噬,流式细胞仪(FCM)检测用药后细胞周期及凋亡率.结果 低水平(1、5 μmol/L)厄洛替尼作用下两种细胞MAP1-LC3-Ⅱ表达水平较对照组(0.1% DMSO)均升高,高水平(25 μmol/L)厄洛替尼作用下A549细胞MAP1-LC3-Ⅱ表达水平较低水平时降低,而A549/ER细胞MAP1-LC3-Ⅱ表达水平较低水平时仍升高.厄洛替尼水平提高至100 μmol/L可诱导A549/ER细胞凋亡,3-MA抑制自噬后A549/ER细胞的凋亡率从7.76%上升至18.85%.结论 自噬增强了A549/ER的耐厄洛替尼作用,抑制自噬可增强厄洛替尼的抗肿瘤作用. 【总页数】4页(1324-1326,1329) 【关键词】肺肿瘤;自噬;厄洛替尼;细胞凋亡 【作者】徐睿;孙建国;陈正堂
【作者单位】第三军医大学附属新桥医院全军肿瘤研究所,重庆,400037 【正文语种】中文 【中图分类】 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_chongqing-medicine_thesis/0201231375220.html 【相关文献】
1.自噬在厄洛替尼诱导的肺腺癌细胞A549凋亡中的作用 [J], 徐磊; 赵松; 党丽峰; 齐宇; 杨洋; 刘东雷; 吴恺; 王啸林; 鲍培龙
2.厄洛替尼对人肺腺癌A549细胞的放射增敏作用 [J], 刘小群; 乔田奎
3.雷帕霉素对顺铂作用下人肺腺癌A549及耐药A549／DDP细胞增殖、侵袭、黏附及自噬凋亡的影响 [J], 金柱; 高宝安
4.厄洛替尼联合RNAi对人肺腺癌A549细胞凋亡及survivin表达的影响 [J], 谷蕾; 吕喜英
5.厄洛替尼联合Survivin siRNA对人肺腺癌细胞A549增殖的影响 [J], 林萍萍; 谷蕾; 吕喜英
以上内容为文献基本信息，获取文献全文请下载
自噬在A549肺腺癌细胞耐厄洛替尼中的作用研究
