二烯丙基二硫抑制人胃癌SGC7901细胞侵袭及相关机制
谷彬燕;向姝霖;庄英帜;姜浩;夏红;苏琦
【期刊名称】《中国药理学通报》 【年(卷),期】2012(028)002
【摘要】Aim To investigate the effects of migration and invasion and its molecular mechanism in human gastric cancer SGC7901 cells induced by diallyl disul-fide ( DADS ). Methods The effects of DADS for migration and invasion were examined by Wound Healing assay and Transwell assay, and the expression of mRNA and protein of MMP-9 and TIMP-3 were determined by RT-PCR and Western blot in SGC7901 cells, respectively. Results Wound Healing assay discovered that the scuffing distance of SGC7901 cells exposed to 15 mg · L-1 DADS for 12 h and 24 h was significantly increased compared with no-treatment group, indicating DADS could inhibit migration in SGC7901 cells ( P < 0. 05 ). Transwell assay showed that the number of SGC7901 cells migrated through the Matrigel treated by 15 mg· L-1 DADS for 12 h and 24 h was 31. 9 ±0. 46 and 23. 5 ± 0. 52 respectively, obviously decreased compared with 51. 6 ± 0. 68 and 41. 3 ± 0. 72 of no-treatment group, respectively, indicating DADS could inhibit invasion in SGC7901 cells ( P < 0. 05 ). RT-PCR showed that expression of MMP-9 mRNA was down-regulated and expression of TIMP-3 mRNA was up-regulated in SGC7901 cells treated by 15 mg · L-1 DADS for 2 h and 24 h,
二烯丙基二硫抑制人胃癌SGC7901细胞侵袭及相关机制
