Effect of Phyllanthus emblica Linn. on candida adhesion to oral epithelium and denture acrylic

Effect of Phyllanthus emblica Linn. on candida adhesion to oral epithelium and denture acrylic

Boonyanit Thaweboon;Sroisiri Thaweboon

【期刊名称】《亚太热带医药杂志（英文版）》 【年(卷),期】2011(000)001

【摘要】Objective: To investigate the effect of Phyllanthus emblica (P. emblica) Linn. ethanolic extract on the adhesion of Candida albicans (C. albicans) to human buccal epithelial cells (BECs) and denture acrylic surfaces. Methods: Human BECs and transparent acrylic strips were pretreated with ethanolic extract solution of P. emblica fruits at concentration ranged from 18.7 to 300 mg/mL. After washing BECs and the strips were inoculated with three strains of C. albicans (ATCC 10281 and two clinical isolates) (107 cells/mL). Normal saline solution (NSS) and 0.2%chlorhexidine gluconate were used as negative and positive controls, respectively. BECs were harvested on 12 μm-polycarbonate filters (Millipore, USA). The membrane filters and the strips were stained with Gram stain. Adherent yeast cells on 100 randomly selected epithelial cells and 20 randomly selected fields on each strip were counted under microscope. The statistical significance was calculated by Kruskal-Wallis and Tukey tests at a significant level of P< 0.05.Results:Significant lower numbers of all strains of yeasts adhering to BECs and acrylic strips were observed after exposure to 75-300

mg/mL of plant extract compared with NSS. Conclusions:The present study demonstrates that P. emblica ethanolic extract interferes with the adhesion of C.albicans to BECs and denture acrylic surfaces in vitro. 【总页数】5页(41-45) 【关键词】

【作者】Boonyanit Thaweboon;Sroisiri Thaweboon

【作者单位】Department of 0ral Microbiology, Faculty of Dentistry, Mahidol

University,6

Yothi

Road,

Rajthevee,

Bangkok

10400,

Thailand;Department of 0ral Microbiology, Faculty of Dentistry, Mahidol University,6 Yothi Road, Rajthevee, Bangkok 10400, Thailand 【正文语种】英文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_asia-pacific-tropical-medicine-journal-english_thesis/0201254096551.html 【相关文献】

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