脂多糖诱导细胞因子紊乱抑郁症模型小鼠海马蛋白质组学研究

脂多糖诱导细胞因子紊乱抑郁症模型小鼠海马蛋白质组学

研究

唐建勇;周婵娟;吴优;李雯雯;谢鹏

【期刊名称】《中华精神科杂志》 【年(卷),期】2016(049)004

【摘要】Objective To explore the differential proteomics of hippocampal tissue proteins and the pathogenesis in a mice depression model which induced by lipopolysaccharide (LPS).Methods According to the baseline of sucrose consumption and weight,60 male Charles River Cancer Research CD-I(R) (ICR) mice were randomly divided into depression model group and control groups (n=30 per group).After establishment of mice depression model by intraperitoneal injection of LPS,the tissue proteins

were

extracted

from

hippocampus

of

both

groups.Subsequently,the differential proteomics analysis wasperformed by conventional two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF-MS),and Western blotting was used to protein verification.Results (1)The experimental results from the 24 h sucrose consumption test showed consistent decreases of liquid intake (t=-8.529,P=0.008) and sucrose preference (t=-9.463,P=0.006) in the model group,forced swimming test immobility (t=3.105,P=0.003) and tail suspension test immobility (t=3.228,P=0.002) results showed