Inter-and Intra-specific Genetic Diversity in Diospyros Using SCoT and IRAP Markers

Inter-and Intra-specific Genetic Diversity in Diospyros Using SCoT and IRAP Markers

Changfei Guan; Sadaruddin Chachar; Pingxian Zhang; Chaoqiong Hu; RenziWang; Yong Yang

【期刊名称】《园艺学报：英文版》 【年(卷),期】2020(006)002

【摘要】The genus Diospyros contains more than 500 species, among them persimmon(Diospyros kaki Thunb.) is the most economically important species. However, the inter-and intra-specific genetic diversity remains unknown due to historic cultivation, domestication and introduction in different regions of China. In this study, we investigated the genetic diversity and relationship among 268 Diospyros accessions from different regions in China using start codon-targeted(SCo

T)

and

inter-retrotransposon-amplified

polymorphism(IRAP) markers. Among 268 samples,232 accessions were D. kaki varieties and 36 were closely related species, that is, D. lotus(21), D. glaucifolia(1), D. virginiana(5), D. kaki Thunb.var. silvestris(3), D. oleifera(5) and D. deyangnsis(1). In our study, a total of 90 and 97 polymorphic alleles were obtained from nine SCo T and nine IRAP markers, respectively, and these markers displayed rich polymorphisms for evaluation of the genetic diversity among 268 samples. A study on the relationship, genetic structure and analysis of molecular