Effects of TRPC6 on invasibility of low-differentiated prostate cancer cells

Effects of TRPC6 on invasibility of low-differentiated prostate cancer cells

Dong Wang;Xiang Li;Jing Liu;Jun Li;Li-Jun Li;Ming-Xing Qiu

【期刊名称】《亚太热带医药杂志（英文版）》 【年(卷),期】2014(000)001

【摘要】Objective: To study the expression of TRPC6 among prostate cancer cells, establish high expression cell lines of TRPC6, and to provide potential cell mode for prostate cancer oncogenesis and development. Methods: Occurrence and development of prostate cancer cells, PC3, PC-3 m DU145, 22 rv1, LNCaP and normal prostate epithelial cells in the PrEC TRPC6 expression level were detected by QPCR method. Calcium phosphate transfection method was used to package retrovirus pLEGFP-N1-TRPC6 and pLEGFP-N1-vector and infect the prostate cancer cells, a stable high expression of TRPC6 prostate cancer cells. Sable cell lines of TRPC6, matrix metalloproteinase (MMP) 2, MMP9 expression was detected by QPCR and Western blot. Change of cell invasion ability was detected by Transwell. Results: The expression level of prostate cancer cells TRPC6 were higher than control group PrEC cells. Among TPRC6 the expression of cell line PC 3 transfer potential wre the lowest, and high transfer cell line PC-3M express was the highest. Real-time fluorescent quantitative PCR and western blot results showed that after filter, the seventh generation of cell TRPC6

protein and mRNA expression levels were higher than the control group obviously.

Transwell

experimental

results

showed

that

the

overexpression of TRPC6 could promote the invasion ability of PC3 prostate cancer cells. Conclusions: TRPC6 expressed in prostate cancer cells is in disorder, and its action may be associated with the invasion and metastasis of prostate cancer cells; successful establishment of stable high expression of TRPC6 prostate cancer cells primarily confirm the invasion-trigger ability of TRPC6 on prostate cancer, and lay down the foundation for exploring the TRPC6’s role in the occurrence and development of prostate cancer mechanism. 【总页数】4页(44-47) 【关键词】

【作者】Dong Wang;Xiang Li;Jing Liu;Jun Li;Li-Jun Li;Ming-Xing Qiu 【作者单位】Department of Urology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China;Department of Urology, West China Hospital of Sichuan University, Chengdu 610041, China;Department of Urology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China;Department of Urology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China;Department of Urology, Sichuan Academy of Medical Sciences & Sichuan

Provincial

People's

Hospital,

Chengdu

610072,

China;Department of Urology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, China 【正文语种】英文 【中图分类】 【文献来源】

https://www.zhangqiaokeyan.com/academic-journal-cn_asia-pacific-tropical-medicine-journal-english_thesis/0201254094239.html 【相关文献】

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