Fusion expression of mutated cecropin CMIV in E.
coli
作者：谢维;邱奇峰;董雪吟;华子春;徐贤秀;;
作者机构：State Key Laboratory of Pharmaceutical
Biotechnology;Department of Biochemistry;Nanjing University;Nanjing 210093;China;China; 来源：中国科学 年：1997 卷：000 期：003
页码：P.225-231 页数：7 中图分类：Q78 正文语种：CHI
关键词：cecropin fusion expression E. coli.
摘要：A cDNA coding mutated cecropin CMIV from Bombyx mori was synthesized according to its amino acid sequense using E .coli biased codons .The gene was cloned into the fusion expression vector pEZZ318 and was expressed in E .coli HB101.The fusion protein produced was purified by affinity chromatography to yield 26 mg/L fusion product .The anti-bacterial activities of recombinant cecropin CMIV were recovered after cleavage by chemical method.
Fusion expression of mutated cecropin CMIV in E. coli
