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ＨＰＬＣ法测定华法林片的含量
作者：黄碧莹 姚秋燕 李敏薇 任 斌 林婉贞 来源：《中国实用医药》2008年第25期
【摘要】 目的 建立HPLC法测华法林片的含量。方法 采用Diamonsil C18(4．6 mm×250 mm,5 μm)为分离柱；流动相：乙腈-水-冰醋酸(70∶30∶1)；流速：1 ml/min；检测波长308 nm，以峰面积计算。结果 线性范围：0．8~5．6 μg/ml，r=0．999 6。平均回收率为
100．64%，RSD=1．11%,n=15。结论 本法简便，快捷，结果准确，重现性好，可作为华法林片质量控制的有效方法。
【关键词】High performance liguid chromatography ；华法林；含量测定
Determination of warfarin in tablets by HPLC
HUANG Bi-ying,YAO Qiu-yan,LI Min-wei,et al.Dept of Pharmacy,the First Affiliated Hospital of Sun Yat-sen University,Guangdong,Guangzhou 510080，China
【Abstract】 Objective To establish a HPLC method to measure the content of warfarin tablets.Methods A Diamonsil C18column(4．6 mm×250 mm,5 μm)was used.The mobile phase was a mixture of 70?etonitrile,30%water and 1%glacial acetic acid,and the flow rate was
1．0ml/min.The detection wavelength was at 308 nm.Results The linear calibration curves were obtained over the range 0．8 μg/mlto 5．6 μg/ml of warfarin,r=0．999 6．The recovery of the assay was 100．64%(n=15).The coefficients of variation was 1．11%.Conclusion The method appeared to
【Key words】HPLC；Warfarin；Determination
华法林(warfarin)为香豆素类口服抗凝药，化学结构与维生素K相似，可竞争性拮抗维生素K的作用。临床上用于防治血栓栓塞性疾病及作为心肌梗死的辅助用药[1]。本文参考文献[2]建立了HPLC法测定华法林的含量，试验结果表明，该法简便，快捷，准确，重现性好。
HPLC法测定华法林片的含量
