川芎嗪对新生鼠缺血缺氧脑损伤HIF-1α表达的影响
李娟;俞海国;陆亚东
【期刊名称】《南京医科大学学报（自然科学版）》 【年(卷),期】2009(029)011
【摘要】Objective: To explore the effect of liguistrazine on the expression of hypoxia-induced factor-1α(HIF-1α)in neonatal rats with hypoxic-ischemic brain damage (HIBD). Methods: Models of 7-day-old neonatal rats with HIBD were established by occluding the left common carotid artery and subjected to inhalation of 8% oxygen. The mice of treated group were injected with liguistrazine intraperitoneally (50 mg/kg) before and after the operation. The expression of HIF-1α in cortex was measured by RT-PCR and im-munohistochemical staining. Results: Although the levels of HIF-1α mRNA and protein expression at 2 h after HIBD were higher than those of sham group,there was no significance (P > 0.05) .While the expression of HIF-1α mRNA and protein were remarkably higher in liguistrazine treated group than those of HIBD group (P < 0.05). Conclusion: Liguistrazine can increase the expression of HIF-α mRNA and protein in neonatal rats with HIBD,which may be the molecular mechanism of its neuroprotective effect.%目的:探讨实验性大鼠缺血缺氧脑损伤(HIBD)时低氧诱导因子-1α(HIF-1α)的表达及川芎嗪对HIF-1α表达的影响.方法:新生SD大鼠结扎左侧颈总动脉并置于缺氧环境中,制备HIBD模型.川芎嗪干预组在缺血前和缺血后腹腔注射
川芎嗪(50 mg/kg).应用逆转录PCR和免疫组化方法观察川芎嗪对HIBD新生鼠皮质部位HIF-1α mRNA及蛋白表达的影响.结果:缺血缺氧2 h后HIF-1α mRNA及蛋白表达有增加的趋势,但与假手术组相比无统计学意义(P>0.05);川芎嗪干预组HIF-1αmRNA及蛋白表达显著增加,明显高于缺血缺氧组(P<0.05).结论:川芎嗪促进新生鼠缺血缺氧脑损伤皮质部位HIF-1α mRNA及蛋白表达,是其神经保护作用的重要机制. 【总页数】3页(1542-1544)
【关键词】新生鼠;缺血缺氧脑损伤;川芎嗪;低氧诱导因子 【作者】李娟;俞海国;陆亚东
【作者单位】南京医科大学附属南京儿童医院儿科,江苏,南京,210008;南京医科大学附属南京儿童医院儿科,江苏,南京,210008;南京医科大学附属南京儿童医院儿科,江苏,南京,210008 【正文语种】中文 【中图分类】R285.5 【文献来源】
https://www.zhangqiaokeyan.com/academic-journal-cn_journal-nanjing-medical-university-natural-sciences_thesis/0201245463126.html 【相关文献】
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川芎嗪对新生鼠缺血缺氧脑损伤HIF-1α表达的影响
