HPLC测定安神颗粒中酸枣仁皂苷A及B的含量
尚磊;窦强
【期刊名称】《食品与药品》 【年(卷),期】2012(014)006
【摘要】Objective To establish a method for the determination of jujuboside A and B in Anshen Granules and to provide the quality control standard for preparation. Methods Jujuboside A and B in Anshen Granules were determined by high performance liquid chromatography-evaporative light-scattering detector(HPLC-ELSD). The column was Kromasil C18 (4.6 mm ×200 mm,5μm) and the mobile phase consisted of methanol-water-glacial acetic acid (65:35:1) with the flow rate of 1.0 mL/min. The column temperature was 25℃ and the nitrogen flow rate was 2.0 L/min and the temperature of the drift tube was 94 ℃. Results The average recovery of jujuboside A and B were 100.2 % (RSD 1.21 %)和99.88 % (RSD 1.32 %) , respectively. Conclusion The method is simple, accurate and reliable for the quality control of Anshen Granules.%目的 建立测定安神颗粒中酸枣仁皂苷A及B含量的方法,提供该制剂质量控制标准.方法 应用高效液相色谱法-蒸发光散射检测器(HPLC-ELSD),Kromasil C18色谱柱(4.6 mm×200 mm,5 μm),流动相:甲醇-水-冰醋酸(65∶35∶1),流速:1.0 mL/min,柱温25℃,氮气流速2.0 L/min,漂移管温度94℃,测定安神颗粒中酸枣仁皂苷A和B含量.结果 酸枣仁皂苷A及B平均回收率分别为100.2％ (RSD=1.21％)和99.88％(RSD=1.32％).结论 此方法操作简便,结
HPLC测定安神颗粒中酸枣仁皂苷A及B的含量
