Preliminary study on Herpes simplex virus type 1 infection of human oral epithelial cell in vitro

Preliminary study on Herpes simplex virus type 1 infection of human oral epithelial cell in vitro

Jie Zhao; Weibin Sun; Juan Wang

【期刊名称】《《生物医学研究杂志（英文版）》》 【年(卷),期】2008(022)001

【摘要】Objective: To explore the functions and mechanisms of herpes simplex virus type 1(HSV-1) while infecting human oral epithelial cells in vitro(being similar to the infection in vivo). Methods:An abundance of HSV-1 strains amplified in Vero cells were used to infect human oral epithelial cells. The culture supernatant was collected to infect Veto cells again. Morphology of HSV-1 was identified by inverted microscope and transmission electron microscope. Nucleic acid of the virus was detected by PCR. Results:The infected human oral epithelial cells didn't display

an

obvious

cytopathic

effect(CPE)

under

inverted

microscope(while Veto cells which were infected by the culture supematant showed typical(CPE). The virus particles were not observed in the cytoplasm nor in nucleus of human oral epithelial cells, however under transmission electron microscope in the cytoplasm of Vero cells, the nucleic acid of HSV-1 could be detected in infected human oral epithelial cells, by PCR. Conclusion:HSV-1 can successfully infect human oral epithelial cells. This model may provide a useful approach for studying the pathogenesis of herpes virus-associated periodontal